Key Publications

Documentation and Validation

• EPA Environmental Technology Verification
• International Standard Method – ISO 16000:22-2025
  “Detection and quantification of fungal biomass by fungal β-N- acetylhexosaminidase enzyme activity”.
• American Standard Method – ASTM D8527
  Standard Test Method for Assessment of Fungal Biomass on Surfaces or   Coatings by Measuring Fungal β-N-acetylhexosaminidase (NAHA) Activity”.

DOD Preferred Instrumentation

• Institute of Medicine
  “Damp Indoor Spaces and Health” 2004.
• R. Brandys
  “Post Remediation Testing and Verification for Mold and Bacteria” 3rd Edition, 2010, 4th Edition, 2011.
• ASTM Standard D7338-10
  “Standard Guide for Fungal Assessment in Buildings”, 2010.

ASHRAE Innovation Award

• Danish Building Research Institute
  “Instruction on Mould in Buildings – Investigation and Evaluation SBI-274”.
• Litigation acceptance in Texas, California, Virginia and Florida.

The Technology

• The use of fluorogenic substrates to measure fungal presence and activity in soil.
  Appl. Environ. Microbiol. 64:613-617.Miller, M., A. Palojärvi, A. Rangger, M. Reeslev, A. Kjøller. 1998.

• The Mycometer™-test: A new rapid method for detection and quantification of mold in buildings.
  Proceedings of Healthy Buildings 2000, Vol. 1, p.589-590. Reeslev, M. and M. Miller. 2000.
• Quantifying Mold Biomass on Gypsum Board: Comparison of Ergosterol and Beta-N-Acetylhexosaminidase as Mold Biomass Parameters.
  Applied and Environmental Microbiology. Vol. 69, No.7, p. 3996-3998. Reeslev M, Miller M, Nielsen KF. 2003.
• Analytical Instrument Performance Criteria: Application of a Fluorometric Method for the Detection of Mold in Indoor Environments.
  Applied Occupational and Environmental Hygiene. Vol. 18, No.7, p. 499-503. Krause JD, Hamad YY, Ball LB. 2003.

The Technology Used in the Field

• NAGase activity in airborne biomass dust and relationship between NAGase concentrations and fungal spores.
  Aerobiologia Vol. 19, 97 – 105. A.M., Madsen. 2003.
• Application of a Fluorometric Method for the Detection of Mold in Indoor Environments.
  2003, David Krause. Applied Occupational and Environmental Hygiene Volume 18(7): 1–5.
• Successful Mold Growth Remediation in HVAC Systems.
  2008, Paul Buckmaster, Occupational Health and Safety, January.
• Evaluating the level of Airborne Fungi in Buildings by Measurement of Enzyme Activity.
  AIHce Abstracts, Reeslev, M., Nielsen J. C. and Rogers L. 2009.
• Assessing the Bacterial load After Flooding and Sewage Spills and Remediation Efficacy Using Fluorometric Detection of Bacterial hydrolase Activity.
  AIHce Abstracts, Reeslev, M., Nielsen J. C. and Rogers L. 2009.
• Airborne Fungal and Bacterial Components in PM1 Dust from Biofuel Plants.
  Madsen et al. 2009 The Annals of Occupational Hygiene 1-9.
• Airborne enzyme measurements to detect indoor mould exposure.
  Journal of Environmental Monitoring, V.12, p.2161–2164. R. Rylander, et al. 2010.
• Fluorometric detection and estimation of fungal biomass on cultural heritage materials.
  Journal of Microbiological Methods 80, 178–182, Mitchell, R. et al (Harvard) 2010.
• Assessment of the Bacterial Contamination and Remediation Efficacy After Flooding Using Fluorometric Detection.
  Journal of ASTM International, Vol. 8 Issue 10. Reeslev, Nielsen, Rogers. 2011.
• Aggressive Sampling – Improving the Predictive value Of Air Sampling For Fungal Aerosols.
  Proceedings of Indoor Air. 1050. Reeslev M., Nielsen J.C., Miller M. and Rogers L. 2011
• Airborne enzyme in homes of patients with sarcoidosis.
  Terčelj M, Salobir B, Rylander R. 2011 Env Health; 10; 8-13.
• Nocturnal asthma and domestic exposure to fungi.
  Terčelj M, Salobir B, Narancsik Z, Kriznar K, Grzetic-Romcevic T, Matos T, Rylander. R. Indoor+Built Env., 2012.
• Airborne fungal cell fragments in homes in relation to total fungal biomass.
  Indoor Air 23:142-147. Adhikan A, Reponen T. Rylander R. 2013.
• Surface and passive/active air mould sampling: A testing exercise in North London housing estate.
  Yasemin Didem Aktas, Ioanna Ioannou, Hector Altamirano, Morten Reeslev, Dina D’Ayala Neil May, Melisa Canales. Science of the Total Environment 643 (2018) 1631–1643. 2018.
• Normal background levels of air and surface mould reserve in English residential building stock: a preliminary study towards benchmarks based on NAHA measurements.
  Yasemin D. Aktas,* , M. Reeslev; H. Altamirano, N. May, and D. D’Ayala. 2020 UCL Open: Environment.
• Monitored conditions in wooden wall plates in relation to mold and wood decaying fungi.
  Tessa Kvist Hansen1*, Nickolaj Feldt Jensen, Eva Møller, Ernst Jan de Place Hansen, and Ruut Peuhkuri. E3S Web of Conferences 172, 20004. 2020.
• Hygrothermal assessment of diffusion open insulation systems for interior retrofitting of solid masonry walls.
  N.F. Jensen, T.R. Odgaard, S.P. Bjarløv, B. Andersen, C. Rode, E.B. Møller. Building and Environment, Volume 182, September 2020, 107011.
• Laboratory-based investigation of the materials’ water activity and pH relative to fungal growth in internally insulated solid masonry walls.
  Nickolaj Feldt Jensen1, Søren Peter Bjarløv1, Carsten Rode1, Birgitte Andersen2, Eva B. Møller2. 2021 Indoor Air, 31(4), 1252–1266.
• Mind the gap between non-activated (non-aggressive) and activated (aggressive) indoor fungal testing: impact of pre-sampling environmental settings on indoor air readings.
  Spyros Efthymiopoulos a,b,*, Yasemin D. Aktas a,b, Hector Altamirano. 9 April 2022. UCL OPEN Environment journal.